8TP6
H2 hemagglutinin (A/Singapore/1/1957) in complex with RBS-targeting Fab 4-1-1E02
Summary for 8TP6
| Entry DOI | 10.2210/pdb8tp6/pdb |
| EMDB information | 41468 |
| Descriptor | Hemagglutinin, Heavy chain of 4-1-1E02 Fab, Light chain of 4-1-1E02 Fab, ... (6 entities in total) |
| Functional Keywords | influenza, hemagglutinin, monoclonal antibody, immune complex, viral protein, viral protein-immune system complex, viral protein/immune system |
| Biological source | Influenza A virus (A/Singapore/1/1957(H2N2)) More |
| Total number of polymer chains | 9 |
| Total formula weight | 250815.66 |
| Authors | Yang, Y.R.,Han, J.,Perrett, H.R.,Ward, A.B. (deposition date: 2023-08-04, release date: 2023-10-11, Last modification date: 2025-05-21) |
| Primary citation | Yang, Y.R.,Han, J.,Perrett, H.R.,Richey, S.T.,Rodriguez, A.J.,Jackson, A.M.,Gillespie, R.A.,O'Connell, S.,Raab, J.E.,Cominsky, L.Y.,Chopde, A.,Kanekiyo, M.,Houser, K.V.,Chen, G.L.,McDermott, A.B.,Andrews, S.F.,Ward, A.B. Immune memory shapes human polyclonal antibody responses to H2N2 vaccination. Cell Rep, 43:114171-114171, 2024 Cited by PubMed Abstract: Influenza A virus subtype H2N2, which caused the 1957 influenza pandemic, remains a global threat. A recent phase 1 clinical trial investigating a ferritin nanoparticle vaccine displaying H2 hemagglutinin (HA) in H2-naive and H2-exposed adults enabled us to perform comprehensive structural and biochemical characterization of immune memory on the breadth and diversity of the polyclonal serum antibody response elicited. We temporally map the epitopes targeted by serum antibodies after vaccine prime and boost, revealing that previous H2 exposure results in higher responses to the variable HA head domain. In contrast, initial responses in H2-naive participants are dominated by antibodies targeting conserved epitopes. We use cryoelectron microscopy and monoclonal B cell isolation to describe the molecular details of cross-reactive antibodies targeting conserved epitopes on the HA head, including the receptor-binding site and a new site of vulnerability deemed the medial junction. Our findings accentuate the impact of pre-existing influenza exposure on serum antibody responses post-vaccination. PubMed: 38717904DOI: 10.1016/j.celrep.2024.114171 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.1 Å) |
Structure validation
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