8TO0
48-nm repeating structure of doublets from mouse sperm flagella
This is a non-PDB format compatible entry.
Summary for 8TO0
| Entry DOI | 10.2210/pdb8to0/pdb |
| EMDB information | 41315 41316 41431 |
| Descriptor | Cilia- and flagella-associated protein 95, Cilia- and flagella-associated protein 53, Tektin bundle-interacting protein 1, ... (35 entities in total) |
| Functional Keywords | mammalian sperm, axoneme, microtubule-based structure, microtubule inner protein, non-motor proteins, cellular motility, fertility, structural protein |
| Biological source | Mus musculus (house mouse) More |
| Total number of polymer chains | 471 |
| Total formula weight | 22692158.01 |
| Authors | Chen, Z.,Shiozak, M.,Hass, K.M.,Skinner, W.,Zhao, S.,Guo, C.,Polacco, B.J.,Yu, Z.,Krogan, N.J.,Kaake, R.M.,Vale, R.D.,Agard, D.A. (deposition date: 2023-08-02, release date: 2023-11-01, Last modification date: 2023-11-22) |
| Primary citation | Chen, Z.,Shiozaki, M.,Haas, K.M.,Skinner, W.M.,Zhao, S.,Guo, C.,Polacco, B.J.,Yu, Z.,Krogan, N.J.,Lishko, P.V.,Kaake, R.M.,Vale, R.D.,Agard, D.A. De novo protein identification in mammalian sperm using in situ cryoelectron tomography and AlphaFold2 docking. Cell, 186:5041-5053.e19, 2023 Cited by PubMed Abstract: To understand the molecular mechanisms of cellular pathways, contemporary workflows typically require multiple techniques to identify proteins, track their localization, and determine their structures in vitro. Here, we combined cellular cryoelectron tomography (cryo-ET) and AlphaFold2 modeling to address these questions and understand how mammalian sperm are built in situ. Our cellular cryo-ET and subtomogram averaging provided 6.0-Å reconstructions of axonemal microtubule structures. The well-resolved tertiary structures allowed us to unbiasedly match sperm-specific densities with 21,615 AlphaFold2-predicted protein models of the mouse proteome. We identified Tektin 5, CCDC105, and SPACA9 as novel microtubule-associated proteins. These proteins form an extensive interaction network crosslinking the lumen of axonemal doublet microtubules, suggesting their roles in modulating the mechanical properties of the filaments. Indeed, Tekt5 -/- sperm possess more deformed flagella with 180° bends. Together, our studies presented a cellular visual proteomics workflow and shed light on the in vivo functions of Tektin 5. PubMed: 37865089DOI: 10.1016/j.cell.2023.09.017 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.7 Å) |
Structure validation
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