8TGO

Crystal structure of the BG505 triple tandem trimer gp140 HIV-1 Env in complex with PGT124 and 35O22

これはPDB形式変換不可エントリーです。

8TGO の概要

• エントリーDOI: 10.2210/pdb8tgo/pdb
• 分子名称: Envelope glycoprotein gp41, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose, ... (11 entities in total)
• 機能のキーワード: hiv, envelope, antibody, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 476175.39

構造登録者

Xian, Y.,Yuan, M.,Wilson, I.A. (登録日: 2023-07-12, 公開日: 2024-04-17, 最終更新日: 2024-10-30)

主引用文献

Del Moral-Sanchez, I.,Wee, E.G.,Xian, Y.,Lee, W.H.,Allen, J.D.,Torrents de la Pena, A.,Froes Rocha, R.,Ferguson, J.,Leon, A.N.,Koekkoek, S.,Schermer, E.E.,Burger, J.A.,Kumar, S.,Zwolsman, R.,Brinkkemper, M.,Aartse, A.,Eggink, D.,Han, J.,Yuan, M.,Crispin, M.,Ozorowski, G.,Ward, A.B.,Wilson, I.A.,Hanke, T.,Sliepen, K.,Sanders, R.W.
Triple tandem trimer immunogens for HIV-1 and influenza nucleic acid-based vaccines.
Npj Vaccines, 9:74-74, 2024

PubMed Abstract: Recombinant native-like HIV-1 envelope glycoprotein (Env) trimers are used in candidate vaccines aimed at inducing broadly neutralizing antibodies. While state-of-the-art SOSIP or single-chain Env designs can be expressed as native-like trimers, undesired monomers, dimers and malformed trimers that elicit non-neutralizing antibodies are also formed, implying that these designs could benefit from further modifications for gene-based vaccination approaches. Here, we describe the triple tandem trimer (TTT) design, in which three Env protomers are genetically linked in a single open reading frame and express as native-like trimers. Viral vectored Env TTT induced similar neutralization titers but with a higher proportion of trimer-specific responses. The TTT design was also applied to generate influenza hemagglutinin (HA) trimers without the need for trimerization domains. Additionally, we used TTT to generate well-folded chimeric Env and HA trimers that harbor protomers from three different strains. In summary, the TTT design is a useful platform for the design of HIV-1 Env and influenza HA immunogens for a multitude of vaccination strategies.

PubMed: 38582771
DOI: 10.1038/s41541-024-00862-8

実験手法

X-RAY DIFFRACTION (5.75 Å)