8TB1

Solution NMR structure of a RiPP proteusin precursor protein

Summary for 8TB1

• Entry DOI: 10.2210/pdb8tb1/pdb
• NMR Information: BMRB: 31097
• Descriptor: NHLP leader peptide family natural product, tumor homing peptide 1 (TH1) substrate chimera (1 entity in total)
• Functional Keywords: ripp, proteusin, nhlp, nitrile hdydratase-like protein, biosynthetic protein
• Biological source: Methylovulum psychrotolerans; More
• Total number of polymer chains: 1
• Total formula weight: 9677.90

Authors

McShan, A.C.,Vinayak, A.,Nguyen, N.A. (deposition date: 2023-06-28, release date: 2024-02-14, Last modification date: 2024-05-01)

Primary citation

Nguyen, N.A.,Vidya, F.N.U.,Yennawar, N.H.,Wu, H.,McShan, A.C.,Agarwal, V.
Disordered regions in proteusin peptides guide post-translational modification by a flavin-dependent RiPP brominase.
Nat Commun, 15:1265-1265, 2024

PubMed Abstract: To biosynthesize ribosomally synthesized and post-translationally modified peptides (RiPPs), enzymes recognize and bind to the N-terminal leader region of substrate peptides which enables catalytic modification of the C-terminal core. Our current understanding of RiPP leaders is that they are short and largely unstructured. Proteusins are RiPP precursor peptides that defy this characterization as they possess unusually long leaders. Proteusin peptides have not been structurally characterized, and we possess scant understanding of how these atypical leaders engage with modifying enzymes. Here, we determine the structure of a proteusin peptide which shows that unlike other RiPP leaders, proteusin leaders are preorganized into a rigidly structured region and a smaller intrinsically disordered region. With residue level resolution gained from NMR titration experiments, the intermolecular peptide-protein interactions between proteusin leaders and a flavin-dependent brominase are mapped onto the disordered region, leaving the rigidly structured region of the proteusin leader to be functionally dispensable. Spectroscopic observations are biochemically validated to identify a binding motif in proteusin peptides that is conserved among other RiPP leaders as well. This study provides a structural characterization of the proteusin peptides and extends the paradigm of RiPP modification enzymes using not only unstructured peptides, but also structured proteins as substrates.

PubMed: 38341413
DOI: 10.1038/s41467-024-45593-5

Experimental method

SOLUTION NMR