8TAR

APC/C-CDH1-UBE2C-Ubiquitin-CyclinB-NTD

Summary for 8TAR

• Entry DOI: 10.2210/pdb8tar/pdb
• EMDB information: 41140
• Descriptor: Anaphase-promoting complex subunit 1, Anaphase-promoting complex subunit 10, Anaphase-promoting complex subunit 13, ... (18 entities in total)
• Functional Keywords: e3 ring ligase, ubiquitin ligase, ligase, transferase
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 22
• Total formula weight: 1228921.53

Authors

Bodrug, T.,Welsh, K.A.,Bolhuis, D.L.,Paulakonis, E.,Martinez-Chacin, R.C.,Liu, B.,Pinkin, N.,Bonacci, T.,Cui, L.,Xu, P.,Roscow, O.,Amann, S.J.,Grishkovskaya, I.,Emanuele, M.J.,Harrison, J.S.,Steimel, J.P.,Hahn, K.M.,Zhang, W.,Zhong, E.,Haselbach, D.,Brown, N.G. (deposition date: 2023-06-27, release date: 2023-09-27, Last modification date: 2023-11-22)

Primary citation

Bodrug, T.,Welsh, K.A.,Bolhuis, D.L.,Paulаkonis, E.,Martinez-Chacin, R.C.,Liu, B.,Pinkin, N.,Bonacci, T.,Cui, L.,Xu, P.,Roscow, O.,Amann, S.J.,Grishkovskaya, I.,Emanuele, M.J.,Harrison, J.S.,Steimel, J.P.,Hahn, K.M.,Zhang, W.,Zhong, E.D.,Haselbach, D.,Brown, N.G.
Time-resolved cryo-EM (TR-EM) analysis of substrate polyubiquitination by the RING E3 anaphase-promoting complex/cyclosome (APC/C).
Nat.Struct.Mol.Biol., 30:1663-1674, 2023

PubMed Abstract: Substrate polyubiquitination drives a myriad of cellular processes, including the cell cycle, apoptosis and immune responses. Polyubiquitination is highly dynamic, and obtaining mechanistic insight has thus far required artificially trapped structures to stabilize specific steps along the enzymatic process. So far, how any ubiquitin ligase builds a proteasomal degradation signal, which is canonically regarded as four or more ubiquitins, remains unclear. Here we present time-resolved cryogenic electron microscopy studies of the 1.2 MDa E3 ubiquitin ligase, known as the anaphase-promoting complex/cyclosome (APC/C), and its E2 co-enzymes (UBE2C/UBCH10 and UBE2S) during substrate polyubiquitination. Using cryoDRGN (Deep Reconstructing Generative Networks), a neural network-based approach, we reconstruct the conformational changes undergone by the human APC/C during polyubiquitination, directly visualize an active E3-E2 pair modifying its substrate, and identify unexpected interactions between multiple ubiquitins with parts of the APC/C machinery, including its coactivator CDH1. Together, we demonstrate how modification of substrates with nascent ubiquitin chains helps to potentiate processive substrate polyubiquitination, allowing us to model how a ubiquitin ligase builds a proteasomal degradation signal.

PubMed: 37735619
DOI: 10.1038/s41594-023-01105-5

Experimental method

ELECTRON MICROSCOPY (4 Å)