8T79

SpRY-Cas9:gRNA complex bound to non-target DNA with 10 bp R-loop

Summary for 8T79

• Entry DOI: 10.2210/pdb8t79/pdb
• EMDB information: 41088
• Descriptor: CRISPR-associated endonuclease Cas9/Csn1, gRNA, TS, ... (4 entities in total)
• Functional Keywords: spry-cas9, crispr, cas9, immune system, r-loop
• Biological source: Streptococcus pyogenes; More
• Total number of polymer chains: 4
• Total formula weight: 196246.89

Authors

Hibshman, G.N.,Bravo, J.P.K.,Taylor, D.W. (deposition date: 2023-06-20, release date: 2024-05-08)

• Primary citation: Hibshman, G.N.,Bravo, J.P.K.,Hooper, M.M.,Dangerfield, T.L.,Zhang, H.,Finkelstein, I.J.,Johnson, K.A.,Taylor, D.W.; Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9.; Nat Commun, 15:3663-3663, 2024; PubMed: 38688943; DOI: 10.1038/s41467-024-47830-3

Experimental method

ELECTRON MICROSCOPY (3.04 Å)