8SY7

E. coli DNA-directed RNA polymerase transcription elongation complex bound the unnatural dB-STP base pair in the active site

Summary for 8SY7

Entry DOI	10.2210/pdb8sy7/pdb
EMDB information	40864
Descriptor	DNA-directed RNA polymerase subunit alpha, [[(2~{R},3~{S},4~{R},5~{S})-5-(4-azanyl-1-methyl-2-oxidanylidene-pyrimidin-5-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate, DNA-directed RNA polymerase subunit beta, ... (11 entities in total)
Functional Keywords	complex, unnatural base pairs, synthetic biology, transcription, transcription-dna-rna complex, transcription/dna/rna
Biological source	Escherichia coli More
Total number of polymer chains	8
Total formula weight	410283.88
Authors	Shan, Z.,Lyumkis, D.,Oh, J.,Wang, D. (deposition date: 2023-05-24, release date: 2023-12-27)
Primary citation	Oh, J.,Shan, Z.,Hoshika, S.,Xu, J.,Chong, J.,Benner, S.A.,Lyumkis, D.,Wang, D. A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E. coli RNA polymerase. Nat Commun, 14:8219-8219, 2023 Cited by PubMed: 38086811 DOI: 10.1038/s41467-023-43735-9 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (2.65 Å)

Structure validation