8SWS

Structure of K. lactis PNP S42E-H98R variant bound to transition state analog DADMe-IMMUCILLIN G and sulfate

Summary for 8SWS

Entry DOI	10.2210/pdb8sws/pdb
Related	8SWP 8SWQ 8SWR 8SWT 8SWU
Descriptor	Purine nucleoside phosphorylase, 2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one, SULFATE ION, ... (4 entities in total)
Functional Keywords	pentosyltransferase, purine nucleoside phosphorylase, transferase
Biological source	Kluyveromyces lactis NRRL Y-1140
Total number of polymer chains	6
Total formula weight	203415.17
Authors	Fedorov, E.,Ghosh, A. (deposition date: 2023-05-19, release date: 2023-10-18, Last modification date: 2024-02-14)
Primary citation	Minnow, Y.V.T.,Schramm, V.L.,Almo, S.C.,Ghosh, A. Phosphate Binding in PNP Alters Transition-State Analogue Affinity and Subunit Cooperativity. Biochemistry, 62:3116-3125, 2023 Cited by PubMed: 37812583 DOI: 10.1021/acs.biochem.3c00264 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.99 Å)

Structure validation