8SWS

Structure of K. lactis PNP S42E-H98R variant bound to transition state analog DADMe-IMMUCILLIN G and sulfate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E...	Purine nucleoside phosphorylase	polymer	307	33556.2	6	UniProt (Q6CSZ6) Pfam (PF01048) In PDB	Kluyveromyces lactis NRRL Y-1140
2	A, B, D, E	2-amino-7-{[(3R,4R)-3-hydroxy-4-(hydroxymethyl)pyrrolidin-1-yl]methyl}-3,5-dihydro-4H-pyrrolo[3,2-d]pyrimidin-4-one	non-polymer		279.3	4	Chemie (IM5)
3	A, B, D, E, F	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
4		water	water		18.0	313	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 306 (UniProt: Q6CSZ6)

PDB	External Database	Details
Ser 0	-	expression tag
Glu 42	Ser 42	engineered mutation
Arg 98	His 98	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	201337.4
Non-Polymers*	Number of molecules	14
	Total formula weight	2077.8
All*	Total formula weight	203415.2

*Water molecules are not included.