8SW8
Crystal Structure of HaloTag7 bound to JF669-HaloTag ligand
This is a non-PDB format compatible entry.
Summary for 8SW8
| Entry DOI | 10.2210/pdb8sw8/pdb |
| Related | 6U32 6Y7A 6Y7B |
| Descriptor | Haloalkane dehalogenase, 1-[(4aM,10P)-7-(azetidin-1-yl)-10-[2-carboxy-3,4,6-trifluoro-5-({2-[2-(hexyloxy)ethoxy]ethyl}carbamoyl)phenyl]-5,5-dimethyldibenzo[b,e]silin-3(5H)-ylidene]azetidin-1-ium, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | halotag, self-labeling protein, fluorescent dye, hydrolase |
| Biological source | Rhodococcus sp. (in: high G+C Gram-positive bacteria) |
| Total number of polymer chains | 1 |
| Total formula weight | 35288.71 |
| Authors | Farrants, H.,Schreiter, E.R. (deposition date: 2023-05-17, release date: 2024-06-05, Last modification date: 2024-10-23) |
| Primary citation | Farrants, H.,Shuai, Y.,Lemon, W.C.,Monroy Hernandez, C.,Zhang, D.,Yang, S.,Patel, R.,Qiao, G.,Frei, M.S.,Plutkis, S.E.,Grimm, J.B.,Hanson, T.L.,Tomaska, F.,Turner, G.C.,Stringer, C.,Keller, P.J.,Beyene, A.G.,Chen, Y.,Liang, Y.,Lavis, L.D.,Schreiter, E.R. A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging. Nat.Methods, 21:1916-1925, 2024 Cited by PubMed Abstract: Genetically encoded fluorescent calcium indicators allow cellular-resolution recording of physiology. However, bright, genetically targetable indicators that can be multiplexed with existing tools in vivo are needed for simultaneous imaging of multiple signals. Here we describe WHaloCaMP, a modular chemigenetic calcium indicator built from bright dye-ligands and protein sensor domains. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan. WHaloCaMP is compatible with rhodamine dye-ligands that fluoresce from green to near-infrared, including several that efficiently label the brain in animals. When bound to a near-infrared dye-ligand, WHaloCaMP shows a 7× increase in fluorescence intensity and a 2.1-ns increase in fluorescence lifetime upon calcium binding. We use WHaloCaMP1a to image Ca responses in vivo in flies and mice, to perform three-color multiplexed functional imaging of hundreds of neurons and astrocytes in zebrafish larvae and to quantify Ca concentration using fluorescence lifetime imaging microscopy (FLIM). PubMed: 39304767DOI: 10.1038/s41592-024-02411-6 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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