8SW7

BG505 Boost2 SOSIP.664 in complex with NHP polyclonal antibody FP1

This is a non-PDB format compatible entry.

Summary for 8SW7

• Entry DOI: 10.2210/pdb8sw7/pdb
• EMDB information: 40810
• Descriptor: FP1 heavy chain, FP1 light chain, BG505 Boost 2 gp120, ... (6 entities in total)
• Functional Keywords: hiv-1, env, nhp, antibody, fusion peptide, polyclonal, viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Human immunodeficiency virus 1; More
• Total number of polymer chains: 8
• Total formula weight: 258227.42

Authors

Pratap, P.P.,Antansijevic, A.,Ozorowski, G.,Ward, A.B. (deposition date: 2023-05-17, release date: 2023-07-26, Last modification date: 2025-05-21)

Primary citation

Cottrell, C.A.,Pratap, P.P.,Cirelli, K.M.,Carnathan, D.G.,Enemuo, C.A.,Antanasijevic, A.,Ozorowski, G.,Sewall, L.M.,Gao, H.,Greene, K.M.,Allen, J.D.,Ngo, J.T.,Choe, Y.,Nogal, B.,Silva, M.,Bhiman, J.,Pauthner, M.,Irvine, D.J.,Montefiori, D.,Crispin, M.,Burton, D.R.,Silvestri, G.,Crotty, S.,Ward, A.B.
Focusing antibody responses to the fusion peptide in rhesus macaques.
Biorxiv, 2023

PubMed Abstract: Immunodominance of antibodies targeting non-neutralizing epitopes and the high level of somatic hypermutation within germinal centers (GCs) required for most HIV broadly neutralizing antibodies (bnAbs) are major impediments to the development of an effective HIV vaccine. Rational protein vaccine design and non-conventional immunization strategies are potential avenues to overcome these hurdles. Here, we report using implantable osmotic pumps to continuously deliver a series of epitope-targeted immunogens to rhesus macaques over the course of six months to elicit immune responses against the conserved fusion peptide. Antibody specificities and GC responses were tracked longitudinally using electron microscopy polyclonal epitope mapping (EMPEM) and lymph node fine-needle aspirates, respectively. Application of cryoEMPEM delineated key residues for on-target and off-target responses that can drive the next round of structure-based vaccine design.

PubMed: 37425865
DOI: 10.1101/2023.06.26.545779

Experimental method

ELECTRON MICROSCOPY (3.73 Å)