8SP5
LINE-1 retrotransposon endonuclease domain complex with Mn2+
Summary for 8SP5
| Entry DOI | 10.2210/pdb8sp5/pdb |
| Descriptor | LINE-1 retrotransposon endonuclease, MANGANESE (II) ION, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | line-1 retrotransposon, endonuclease, metal ion complex, hydrolase |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 2 |
| Total formula weight | 55093.00 |
| Authors | |
| Primary citation | D'Ordine, A.M.,Jogl, G.,Sedivy, J.M. Identification and characterization of small molecule inhibitors of the LINE-1 retrotransposon endonuclease. Nat Commun, 15:3883-3883, 2024 Cited by PubMed Abstract: The long interspersed nuclear element-1 (LINE-1 or L1) retrotransposon is the only active autonomously replicating retrotransposon in the human genome. L1 harms the cell by inserting new copies, generating DNA damage, and triggering inflammation. Therefore, L1 inhibition could be used to treat many diseases associated with these processes. Previous research has focused on inhibition of the L1 reverse transcriptase due to the prevalence of well-characterized inhibitors of related viral enzymes. Here we present the L1 endonuclease as another target for reducing L1 activity. We characterize structurally diverse small molecule endonuclease inhibitors using computational, biochemical, and biophysical methods. We also show that these inhibitors reduce L1 retrotransposition, L1-induced DNA damage, and inflammation reinforced by L1 in senescent cells. These inhibitors could be used for further pharmacological development and as tools to better understand the life cycle of this element and its impact on disease processes. PubMed: 38719805DOI: 10.1038/s41467-024-48066-x PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.23 Å) |
Structure validation
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