8SM1

CRYSTAL STRUCTURE OF HUMAN ANTIBODY 769A9 IN COMPLEX WITH EPSTEIN-BARR VIRUS MAJOR GLYCOPROTEIN GP350

Summary for 8SM1

• Entry DOI: 10.2210/pdb8sm1/pdb
• Descriptor: Envelope glycoprotein gp350, 769A9 Fab heavy chain, 769A9 Fab light chain, ... (6 entities in total)
• Functional Keywords: viral protein, viral protein-immune system complex, viral protein/immune system
• Biological source: Human herpesvirus 4 (Epstein-Barr virus); More
• Total number of polymer chains: 3
• Total formula weight: 96307.95

Authors

Chen, W.-H.,Bu, W.,Cohen, J.I.,Kanekiyo, M.,Joyce, M.G. (deposition date: 2023-04-25, release date: 2024-05-01, Last modification date: 2025-02-26)

Primary citation

Joyce, M.G.,Bu, W.,Chen, W.H.,Gillespie, R.A.,Andrews, S.F.,Wheatley, A.K.,Tsybovsky, Y.,Jensen, J.L.,Stephens, T.,Prabhakaran, M.,Fisher, B.E.,Narpala, S.R.,Bagchi, M.,McDermott, A.B.,Nabel, G.J.,Kwong, P.D.,Mascola, J.R.,Cohen, J.I.,Kanekiyo, M.
Structural basis for complement receptor engagement and virus neutralization through Epstein-Barr virus gp350.
Immunity, 58:295-, 2025

PubMed Abstract: Epstein-Barr virus (EBV) causes infectious mononucleosis and is associated with malignancies in humans. Viral infection of B cells is initiated by the viral glycoprotein 350 (gp350) binding to complement receptor 2 (CR2). Despite decades of effort, no vaccines or curative agents have been developed, partly due to lack of atomic-level understanding of the virus-host interface. Here, we determined the 1.7 Å structure of gp350 in complex with CR2. CR2 binding of gp350 utilized the same set of Arg residues required for recognition of its natural ligand, complement C3d. We further determined the structures of gp350 in complex with three potently neutralizing antibodies (nAbs) obtained from vaccinated macaques and EBV-infected individuals. Like the CR2 interaction, these nAbs targeted the acidic pocket within the CR2-binding site on gp350 using Arg residues. Our results illustrate two axes of molecular mimicry-gp350 versus C3d and CR2 versus EBV nAbs-offering insights for EBV vaccines and therapeutics development.

PubMed: 39909035
DOI: 10.1016/j.immuni.2025.01.010

Experimental method

X-RAY DIFFRACTION (3.29 Å)