8SFP
WT CRISPR-Cas12a with the target strand in the RuvC active site.
Summary for 8SFP
| Entry DOI | 10.2210/pdb8sfp/pdb |
| EMDB information | 40447 |
| Descriptor | CRISPR-associated endonuclease Cas12a, RNA (39-MER), DNA (40-MER), ... (4 entities in total) |
| Functional Keywords | crispr, r-loop, endonuclease, dna binding protein, dna binding protein-dna-rna complex, dna binding protein/dna/rna |
| Biological source | Acidaminococcus sp. BV3L6 More |
| Total number of polymer chains | 4 |
| Total formula weight | 201567.37 |
| Authors | Strohkendl, I.,Taylor, D.W. (deposition date: 2023-04-11, release date: 2024-07-03, Last modification date: 2025-01-22) |
| Primary citation | Strohkendl, I.,Saha, A.,Moy, C.,Nguyen, A.H.,Ahsan, M.,Russell, R.,Palermo, G.,Taylor, D.W. Cas12a domain flexibility guides R-loop formation and forces RuvC resetting. Mol.Cell, 84:2717-2731.e6, 2024 Cited by PubMed Abstract: The specific nature of CRISPR-Cas12a makes it a desirable RNA-guided endonuclease for biotechnology and therapeutic applications. To understand how R-loop formation within the compact Cas12a enables target recognition and nuclease activation, we used cryo-electron microscopy to capture wild-type Acidaminococcus sp. Cas12a R-loop intermediates and DNA delivery into the RuvC active site. Stages of Cas12a R-loop formation-starting from a 5-bp seed-are marked by distinct REC domain arrangements. Dramatic domain flexibility limits contacts until nearly complete R-loop formation, when the non-target strand is pulled across the RuvC nuclease and coordinated domain docking promotes efficient cleavage. Next, substantial domain movements enable target strand repositioning into the RuvC active site. Between cleavage events, the RuvC lid conformationally resets to occlude the active site, requiring re-activation. These snapshots build a structural model depicting Cas12a DNA targeting that rationalizes observed specificity and highlights mechanistic comparisons to other class 2 effectors. PubMed: 38955179DOI: 10.1016/j.molcel.2024.06.007 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.8 Å) |
Structure validation
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