8RWK

cryoEM structure of the central Ald4 filament determined by FilamentID

Summary for 8RWK

• Entry DOI: 10.2210/pdb8rwk/pdb
• EMDB information: 19549
• Descriptor: Potassium-activated aldehyde dehydrogenase, mitochondrial, NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE (2 entities in total)
• Functional Keywords: metabolic enzyme, filament, cryoem, cytosolic protein
• Biological source: Saccharomyces cerevisiae SK1
• Total number of polymer chains: 4
• Total formula weight: 230123.18

Authors

Hugener, J.,Xu, J.,Wettstein, R.,Ioannidi, L.,Velikov, D.,Wollweber, F.,Henggeler, A.,Matos, J.,Pilhofer, M. (deposition date: 2024-02-05, release date: 2024-06-26, Last modification date: 2024-07-03)

Primary citation

Hugener, J.,Xu, J.,Wettstein, R.,Ioannidi, L.,Velikov, D.,Wollweber, F.,Henggeler, A.,Matos, J.,Pilhofer, M.
FilamentID reveals the composition and function of metabolic enzyme polymers during gametogenesis.
Cell, 187:3303-, 2024

PubMed Abstract: Gamete formation and subsequent offspring development often involve extended phases of suspended cellular development or even dormancy. How cells adapt to recover and resume growth remains poorly understood. Here, we visualized budding yeast cells undergoing meiosis by cryo-electron tomography (cryoET) and discovered elaborate filamentous assemblies decorating the nucleus, cytoplasm, and mitochondria. To determine filament composition, we developed a "filament identification" (FilamentID) workflow that combines multiscale cryoET/cryo-electron microscopy (cryoEM) analyses of partially lysed cells or organelles. FilamentID identified the mitochondrial filaments as being composed of the conserved aldehyde dehydrogenase Ald4 and the nucleoplasmic/cytoplasmic filaments as consisting of acetyl-coenzyme A (CoA) synthetase Acs1. Structural characterization further revealed the mechanism underlying polymerization and enabled us to genetically perturb filament formation. Acs1 polymerization facilitates the recovery of chronologically aged spores and, more generally, the cell cycle re-entry of starved cells. FilamentID is broadly applicable to characterize filaments of unknown identity in diverse cellular contexts.

PubMed: 38906101
DOI: 10.1016/j.cell.2024.04.026

Experimental method

ELECTRON MICROSCOPY (3.8 Å)