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8RW5

Symmetry expansion of dimeric transmembrane anti-sigma factor DdvA

Summary for 8RW5
Entry DOI10.2210/pdb8rw5/pdb
EMDB information19404 19543
DescriptorCHAT domain-containing protein (1 entity in total)
Functional Keywordstransmembrane protein, anti-sigma factor, antiviral system, tpr-chat, signaling protein
Biological sourceMyxococcus xanthus
Total number of polymer chains1
Total formula weight108640.59
Authors
Lopez-Alonso, J.P.,Ochoa-Lizarralde, B.,Tascon, I.,Ubarretxena-Belandia, I. (deposition date: 2024-02-02, release date: 2024-11-06)
Primary citationBernal-Bernal, D.,Pantoja-Uceda, D.,Lopez-Alonso, J.P.,Lopez-Rojo, A.,Lopez-Ruiz, J.A.,Galbis-Martinez, M.,Ochoa-Lizarralde, B.,Tascon, I.,Elias-Arnanz, M.,Ubarretxena-Belandia, I.,Padmanabhan, S.
Structural basis for regulation of a CBASS-CRISPR-Cas defense island by a transmembrane anti-sigma factor and its ECF sigma partner.
Sci Adv, 10:eadp1053-eadp1053, 2024
Cited by
PubMed Abstract: How CRISPR-Cas and cyclic oligonucleotide-based antiphage signaling systems (CBASS) are coordinately deployed against invaders remains unclear. We show that a locus containing two CBASS and one type III-B CRISPR-Cas system, regulated by the transmembrane anti-σ DdvA and its cognate extracytoplasmic function (ECF) σ DdvS, can defend against a phage. Cryo-electron microscopy reveals DdvA-DdvS pairs assemble as arrow-shaped transmembrane dimers. Each DdvA periplasmic domain adopts a separase/craspase-type tetratricopeptide repeat (TPR)-caspase HetF-associated with TPR (TPR-CHAT) architecture with an incomplete His-Cys active site, lacking three α-helices conserved among CHAT domains. Each active site faces the dimer interface, raising the possibility that signal-induced caspase-like DdvA autoproteolysis in trans precedes RseP-mediated intramembrane proteolysis and DdvS release. Nuclear magnetic resonance reveals a DdvA cytoplasmic CHCC-type zinc-bound three-helix bundle that binds to DdvS σ and σ domains, undergoing σ-induced helix extension to trap DdvS. Altogether, we provide structural-mechanistic insights into membrane anti-σ-ECF σ regulation of an antiviral CBASS-CRISPR-Cas defense island.
PubMed: 39454004
DOI: 10.1126/sciadv.adp1053
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (2.94 Å)
Structure validation

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PDB entries from 2024-11-06

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