8RTD

Stalk-Arches-IMC structure from the fully-assembled R388 type IV secretion system determined by cryo-EM.

8RTD の概要

• エントリーDOI: 10.2210/pdb8rtd/pdb
• EMDBエントリー: 19488
• 分子名称: TrwJ protein, TrwG protein, TrwE protein, ... (6 entities in total)
• 機能のキーワード: type iv secretion system type 4 secretion system t4ss arches stalk inner membrane complex imc r388 plasmid conjugation bacterial secretion secretion secretion system protein complex virb3 virb4 virb5 virb6 virb8 virb10 trwm trwk trwj trwi trwg trwe, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 34
• 化学式量合計: 1339001.78

構造登録者

Mace, K.,Waksman, G. (登録日: 2024-01-25, 公開日: 2024-06-19, 最終更新日: 2024-08-14)

主引用文献

Mace, K.,Waksman, G.
Cryo-EM structure of a conjugative type IV secretion system suggests a molecular switch regulating pilus biogenesis.
Embo J., 43:3287-3306, 2024

PubMed Abstract: Conjugative type IV secretion systems (T4SS) mediate bacterial conjugation, a process that enables the unidirectional exchange of genetic materials between a donor and a recipient bacterial cell. Bacterial conjugation is the primary means by which antibiotic resistance genes spread among bacterial populations (Barlow 2009; Virolle et al, 2020). Conjugative T4SSs form pili: long extracellular filaments that connect with recipient cells. Previously, we solved the cryo-electron microscopy (cryo-EM) structure of a conjugative T4SS. In this article, based on additional data, we present a more complete T4SS cryo-EM structure than that published earlier. Novel structural features include details of the mismatch symmetry within the OMCC, the presence of a fourth VirB8 subunit in the asymmetric unit of both the arches and the inner membrane complex (IMC), and a hydrophobic VirB5 tip in the distal end of the stalk. Additionally, we provide previously undescribed structural insights into the protein VirB10 and identify a novel regulation mechanism of T4SS-mediated pilus biogenesis by this protein, that we believe is a key checkpoint for this process.

PubMed: 38886579
DOI: 10.1038/s44318-024-00135-z

実験手法

ELECTRON MICROSCOPY (4.33 Å)