8RRS

Structure of mouse RyR2 solubilised in detergent in open state in complex with Ca2+, ATP, caffeine and Nb9657.

これはPDB形式変換不可エントリーです。

8RRS の概要

• エントリーDOI: 10.2210/pdb8rrs/pdb
• EMDBエントリー: 19463
• 分子名称: Ryanodine receptor 2, Nanobody 9657, ADENOSINE-5'-TRIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: ion channel, ca2+, tetramer, transport protein
• 由来する生物種: Vicugna pacos; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 2325873.42

構造登録者

Li, C.,Efremov, R.G. (登録日: 2024-01-23, 公開日: 2024-10-09, 最終更新日: 2024-11-06)

主引用文献

Li, C.,Willegems, K.,Uchanski, T.,Pardon, E.,Steyaert, J.,Efremov, R.G.
Rapid small-scale nanobody-assisted purification of ryanodine receptors for cryo-EM.
J.Biol.Chem., 300:107734-107734, 2024

PubMed Abstract: Ryanodine receptors (RyRs) are large Ca release channels residing in the endoplasmic or sarcoplasmic reticulum membrane. Three isoforms of RyRs have been identified in mammals, the disfunction of which has been associated with a series of life-threatening diseases. The need for large amounts of native tissue or eukaryotic cell cultures limits advances in structural studies of RyRs. Here, we report a method that utilizes nanobodies to purify RyRs from only 5 mg of total protein. The purification process, from isolated membranes to cryo-EM grade protein, is achieved within 4 h on the bench, yielding protein usable for cryo-EM analysis. This is demonstrated by solving the structures of rabbit RyR1, solubilized in detergent, reconstituted into lipid nanodiscs or liposomes, and bovine RyR2 reconstituted in nanodisc, and mouse RyR2 in detergent. The reported method facilitates structural studies of RyRs directed toward drug development and is useful in cases where the amount of starting material is limited.

PubMed: 39233227
DOI: 10.1016/j.jbc.2024.107734

実験手法

ELECTRON MICROSCOPY (3.4 Å)