8RRH

The human prohibitin complex

8RRH の概要

• エントリーDOI: 10.2210/pdb8rrh/pdb
• EMDBエントリー: 19459
• 分子名称: Prohibitin 1, Prohibitin-2 (2 entities in total)
• 機能のキーワード: chaperone, lipid organization, protease regulator, membrane protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 345734.95

構造登録者

Lange, F.,Ratz, M.,Dohrke, J.N.,Wenzel, D.,Riedel, D.,Ilgen, P.,Jakobs, S. (登録日: 2024-01-22, 公開日: 2024-12-18, 最終更新日: 2025-04-02)

主引用文献

Lange, F.,Ratz, M.,Dohrke, J.N.,Le Vasseur, M.,Wenzel, D.,Ilgen, P.,Riedel, D.,Jakobs, S.
In situ architecture of the human prohibitin complex.
Nat.Cell Biol., 2025

PubMed Abstract: Prohibitins are a highly conserved family of proteins that have been implicated in a variety of functions including mitochondrial stress signalling and housekeeping, cell cycle progression, apoptosis, lifespan regulation and many others. The human prohibitins prohibitin 1 and prohibitin 2 have been proposed to act as scaffolds within the mitochondrial inner membrane, but their molecular organization has remained elusive. Here we determined the molecular organization of the human prohibitin complex within the mitochondrial inner membrane using an integrative structural biology approach combining quantitative western blotting, cryo-electron tomography, subtomogram averaging and molecular modelling. The proposed bell-shaped structure consists of 11 alternating prohibitin 1 and prohibitin 2 molecules. This study reveals an average of about 43 prohibitin complexes per crista, covering 1-3% of the crista membrane area. These findings provide a structural basis for understanding the functional contributions of prohibitins to the integrity and spatial organization of the mitochondrial inner membrane.

PubMed: 40119201
DOI: 10.1038/s41556-025-01620-1

実験手法

ELECTRON MICROSCOPY (16.3 Å)