8RO2 の概要
| エントリーDOI | 10.2210/pdb8ro2/pdb |
| 関連するPDBエントリー | 8RO0 8RO1 |
| EMDBエントリー | 19399 |
| 分子名称 | U2 snRNA, Protein BUD31 homolog, Pre-mRNA-splicing factor RBM22, ... (40 entities in total) |
| 機能のキーワード | mrna, splicing, intron lariat spliceosome, pre-mrna |
| 由来する生物種 | Homo sapiens (human) 詳細 |
| タンパク質・核酸の鎖数 | 40 |
| 化学式量合計 | 2326674.78 |
| 構造登録者 | |
| 主引用文献 | Vorlander, M.K.,Rothe, P.,Kleifeld, J.,Cormack, E.D.,Veleti, L.,Riabov-Bassat, D.,Fin, L.,Phillips, A.W.,Cochella, L.,Plaschka, C. Mechanism for the initiation of spliceosome disassembly. Nature, 632:443-450, 2024 Cited by PubMed Abstract: Precursor-mRNA (pre-mRNA) splicing requires the assembly, remodelling and disassembly of the multi-megadalton ribonucleoprotein complex called the spliceosome. Recent studies have shed light on spliceosome assembly and remodelling for catalysis, but the mechanism of disassembly remains unclear. Here we report cryo-electron microscopy structures of nematode and human terminal intron lariat spliceosomes along with biochemical and genetic data. Our results uncover how four disassembly factors and the conserved RNA helicase DHX15 initiate spliceosome disassembly. The disassembly factors probe large inner and outer spliceosome surfaces to detect the release of ligated mRNA. Two of these factors, TFIP11 and C19L1, and three general spliceosome subunits, SYF1, SYF2 and SDE2, then dock and activate DHX15 on the catalytic U6 snRNA to initiate disassembly. U6 therefore controls both the start and end of pre-mRNA splicing. Taken together, our results explain the molecular basis of the initiation of canonical spliceosome disassembly and provide a framework to understand general spliceosomal RNA helicase control and the discard of aberrant spliceosomes. PubMed: 38925148DOI: 10.1038/s41586-024-07741-1 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.5 Å) |
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