8RKF

Crystal structure of the ZP-N1 and ZP-N2 domains of human ZP2 (hZP2-N1N2)

8RKF の概要

• エントリーDOI: 10.2210/pdb8rkf/pdb
• 関連するPDBエントリー: 5BUP 5II6 8RKE 8RKG 8RKH
• EMDBエントリー: 19276 19277
• 分子名称: Zona pellucida sperm-binding protein 2, 2-acetamido-2-deoxy-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: cell adhesion, fertilization, egg-sperm interaction, gamete recognition, sperm receptor, extracellular matrix, egg coat, zona pellucida, glycoprotein, n-glycan, structural protein, zp-n domain, block to polyspermy, post-fertilization cleavage, ovastacin
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 53504.81

構造登録者

Dioguardi, E.,Stsiapanava, A.,de Sanctis, D.,Jovine, L. (登録日: 2023-12-25, 公開日: 2024-03-13, 最終更新日: 2024-11-13)

主引用文献

Nishio, S.,Emori, C.,Wiseman, B.,Fahrenkamp, D.,Dioguardi, E.,Zamora-Caballero, S.,Bokhove, M.,Han, L.,Stsiapanava, A.,Algarra, B.,Lu, Y.,Kodani, M.,Bainbridge, R.E.,Komondor, K.M.,Carlson, A.E.,Landreh, M.,de Sanctis, D.,Yasumasu, S.,Ikawa, M.,Jovine, L.
ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat.
Cell, 187:1440-1459.e24, 2024

PubMed Abstract: Following the fertilization of an egg by a single sperm, the egg coat or zona pellucida (ZP) hardens and polyspermy is irreversibly blocked. These events are associated with the cleavage of the N-terminal region (NTR) of glycoprotein ZP2, a major subunit of ZP filaments. ZP2 processing is thought to inactivate sperm binding to the ZP, but its molecular consequences and connection with ZP hardening are unknown. Biochemical and structural studies show that cleavage of ZP2 triggers its oligomerization. Moreover, the structure of a native vertebrate egg coat filament, combined with AlphaFold predictions of human ZP polymers, reveals that two protofilaments consisting of type I (ZP3) and type II (ZP1/ZP2/ZP4) components interlock into a left-handed double helix from which the NTRs of type II subunits protrude. Together, these data suggest that oligomerization of cleaved ZP2 NTRs extensively cross-links ZP filaments, rigidifying the egg coat and making it physically impenetrable to sperm.

PubMed: 38490181
DOI: 10.1016/j.cell.2024.02.013

実験手法

X-RAY DIFFRACTION (3.2 Å)