8RKF

Crystal structure of the ZP-N1 and ZP-N2 domains of human ZP2 (hZP2-N1N2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.95378
Spacegroup name	P 31 2 1
Unit cell lengths	86.930, 86.930, 178.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.870 - 3.200
R-factor	0.2402
Rwork	0.234
R-free	0.29260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	ZP-N1 domain ensemble from PDB entries 5II6 and D_1292121897
RMSD bond length	0.003
RMSD bond angle	0.692
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.154	3.808
Rmeas	0.163	4.018
Rpim	0.052	1.275
Number of reflections	13385	1296
<I/σ(I)>	11.8	0.6
Completeness [%]	99.3	99.2
Redundancy	9.7	9.8
CC(1/2)	1.000	0.340

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	10-30% (v/v) MPD, 0.1 M MES pH 6.0 or 0.1 M Na-HEPES pH 7.5