8RJB
Structure of the rabbit 80S ribosome stalled on a 2-TMD rhodopsin intermediate in complex with Sec61-RAMP4
This is a non-PDB format compatible entry.
Summary for 8RJB
| Entry DOI | 10.2210/pdb8rjb/pdb |
| EMDB information | 19195 |
| Descriptor | Protein transport protein Sec61 subunit alpha isoform 1, Ribosomal Protein uL30, 60S ribosomal protein L7a, ... (53 entities in total) |
| Functional Keywords | ribosome, membrane protein, translocon, transport |
| Biological source | Bos taurus More |
| Total number of polymer chains | 51 |
| Total formula weight | 2206833.15 |
| Authors | Lewis, A.J.O.,Hegde, R.S. (deposition date: 2023-12-20, release date: 2024-01-24, Last modification date: 2024-08-07) |
| Primary citation | Lewis, A.J.O.,Zhong, F.,Keenan, R.J.,Hegde, R.S. Structural analysis of the dynamic ribosome-translocon complex. Elife, 13:-, 2024 Cited by PubMed Abstract: The protein translocon at the endoplasmic reticulum comprises the Sec61 translocation channel and numerous accessory factors that collectively facilitate the biogenesis of secretory and membrane proteins. Here, we leveraged recent advances in cryo-electron microscopy (cryo-EM) and structure prediction to derive insights into several novel configurations of the ribosome-translocon complex. We show how a transmembrane domain (TMD) in a looped configuration passes through the Sec61 lateral gate during membrane insertion; how a nascent chain can bind and constrain the conformation of ribosomal protein uL22; and how the translocon-associated protein (TRAP) complex can adjust its position during different stages of protein biogenesis. Most unexpectedly, we find that a large proportion of translocon complexes contains RAMP4 intercalated into Sec61's lateral gate, widening Sec61's central pore and contributing to its hydrophilic interior. These structures lead to mechanistic hypotheses for translocon function and highlight a remarkably plastic machinery whose conformations and composition adjust dynamically to its diverse range of substrates. PubMed: 38896445DOI: 10.7554/eLife.95814 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.69243 Å) |
Structure validation
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