8RGE
Serial synchrotron in plate room temperature structure of Lysozyme.
Summary for 8RGE
| Entry DOI | 10.2210/pdb8rge/pdb |
| Descriptor | Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total) |
| Functional Keywords | lysozyme hen egg white, hydrolase |
| Biological source | Gallus gallus (chicken) |
| Total number of polymer chains | 1 |
| Total formula weight | 14389.60 |
| Authors | Thompson, A.J.,Hough, M.A.,Sanchez-Weatherby, J.,Williams, L.J.,Sandy, J.,Worrall, J.A.R. (deposition date: 2023-12-13, release date: 2023-12-27, Last modification date: 2024-11-13) |
| Primary citation | Thompson, A.J.,Sanchez-Weatherby, J.,Williams, L.J.,Mikolajek, H.,Sandy, J.,Worrall, J.A.R.,Hough, M.A. Efficient in situ screening of and data collection from microcrystals in crystallization plates. Acta Crystallogr D Struct Biol, 80:279-288, 2024 Cited by PubMed Abstract: A considerable bottleneck in serial crystallography at XFEL and synchrotron sources is the efficient production of large quantities of homogenous, well diffracting microcrystals. Efficient high-throughput screening of batch-grown microcrystals and the determination of ground-state structures from different conditions is thus of considerable value in the early stages of a project. Here, a highly sample-efficient methodology to measure serial crystallography data from microcrystals by raster scanning within standard in situ 96-well crystallization plates is described. Structures were determined from very small quantities of microcrystal suspension and the results were compared with those from other sample-delivery methods. The analysis of a two-dimensional batch crystallization screen using this method is also described as a useful guide for further optimization and the selection of appropriate conditions for scaling up microcrystallization. PubMed: 38488731DOI: 10.1107/S2059798324001955 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.88 Å) |
Structure validation
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