8RFH

CryoEM structure of the plant helper NLR NRC2 in its resting state

Summary for 8RFH

• Entry DOI: 10.2210/pdb8rfh/pdb
• EMDB information: 19121
• Descriptor: NRC2a, ADENOSINE-5'-DIPHOSPHATE (2 entities in total)
• Functional Keywords: nlr protein, helper nlrl, plant immunity, r-protein, immune system
• Biological source: Nicotiana benthamiana
• Total number of polymer chains: 2
• Total formula weight: 215899.20

Authors

Selvaraj, M.,Kamoun, S.,Contreras, M.P. (deposition date: 2023-12-12, release date: 2024-01-10, Last modification date: 2024-10-30)

Primary citation

Selvaraj, M.,Toghani, A.,Pai, H.,Sugihara, Y.,Kourelis, J.,Yuen, E.L.H.,Ibrahim, T.,Zhao, H.,Xie, R.,Maqbool, A.,De la Concepcion, J.C.,Banfield, M.J.,Derevnina, L.,Petre, B.,Lawson, D.M.,Bozkurt, T.O.,Wu, C.H.,Kamoun, S.,Contreras, M.P.
Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome.
Plos Biol., 22:e3002868-e3002868, 2024

PubMed Abstract: Nucleotide-binding domain and leucine-rich repeat (NLR) proteins can engage in complex interactions to detect pathogens and execute a robust immune response via downstream helper NLRs. However, the biochemical mechanisms of helper NLR activation by upstream sensor NLRs remain poorly understood. Here, we show that the coiled-coil helper NLR NRC2 from Nicotiana benthamiana accumulates in vivo as a homodimer that converts into a higher-order oligomer upon activation by its upstream virus disease resistance protein Rx. The cryo-EM structure of NbNRC2 in its resting state revealed intermolecular interactions that mediate homodimer formation and contribute to immune receptor autoinhibition. These dimerization interfaces have diverged between paralogous NRC proteins to insulate critical network nodes and enable redundant immune pathways, possibly to minimise undesired cross-activation and evade pathogen suppression of immunity. Our results expand the molecular mechanisms of NLR activation pointing to transition from homodimers to higher-order oligomeric resistosomes.

PubMed: 39423240
DOI: 10.1371/journal.pbio.3002868

Experimental method

ELECTRON MICROSCOPY (3.9 Å)