8RF9
CgsiGP1 sample in nanodisc
Summary for 8RF9
| Entry DOI | 10.2210/pdb8rf9/pdb |
| EMDB information | 19116 |
| Descriptor | Cyclic beta 1-2 glucan synthetase (1 entity in total) |
| Functional Keywords | cgs, cyclic glucan, antibiotic |
| Biological source | Agrobacterium tumefaciens |
| Total number of polymer chains | 1 |
| Total formula weight | 313358.66 |
| Authors | Sedzicki, J.,Ni, D.,Lehmann, F.,Stahlberg, H.,Dehio, C. (deposition date: 2023-12-12, release date: 2024-03-20) |
| Primary citation | Sedzicki, J.,Ni, D.,Lehmann, F.,Stahlberg, H.,Dehio, C. Structure-function analysis of the cyclic beta-1,2-glucan synthase from Agrobacterium tumefaciens. Nat Commun, 15:1844-1844, 2024 Cited by PubMed Abstract: The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1,2-glucan (CβG) is a circular polysaccharide critical for host interactions of many bacteria, including major pathogens of humans (Brucella) and plants (Agrobacterium). CβG is produced by the cyclic glucan synthase (Cgs), a multi-domain membrane protein. So far, its structure as well as the mechanism underlining the synthesis have not been clarified. Here we use cryo-electron microscopy (cryo-EM) and functional approaches to study Cgs from A. tumefaciens. We determine the structure of this complex protein machinery and clarify key aspects of CβG synthesis, revealing a distinct mechanism that uses a tyrosine-linked oligosaccharide intermediate in cycles of polymerization and processing of the glucan chain. Our research opens possibilities for combating pathogens that rely on polysaccharide virulence factors and may lead to synthetic biology approaches for producing complex cyclic sugars. PubMed: 38418509DOI: 10.1038/s41467-024-45415-8 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.28 Å) |
Structure validation
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