8REY

Cuniculiplasma divulgatum filament

This is a non-PDB format compatible entry.

Summary for 8REY

• Entry DOI: 10.2210/pdb8rey/pdb
• EMDB information: 19112
• Descriptor: Flagellin-like protein, alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-3)-[D-glycero-beta-D-galacto-heptopyranose-(1-2)]6-deoxy-6-sulfo-beta-D-galacto-heptopyranose-(1-4)-beta-D-glucopyranose-(1-3)-beta-D-galactopyranose (2 entities in total)
• Functional Keywords: cell surface appendage, n-glycosylation, protein fibril
• Biological source: Cuniculiplasma divulgatum
• Total number of polymer chains: 36
• Total formula weight: 575939.12

Authors

Isupov, M.N.,Gaines, M.,Daum, B.,McLaren, M. (deposition date: 2023-12-12, release date: 2025-01-01, Last modification date: 2025-10-01)

Primary citation

Gaines, M.C.,Isupov, M.N.,McLaren, M.,Haque, R.U.,Recalde, A.,Bargiela, R.,Gold, V.A.M.,Albers, S.V.,Golyshin, P.N.,Golyshina, O.V.,Daum, B.
Unusual cell surfaces, pili, and archaella of Thermoplasmatales archaea.
Isme J, 19:-, 2025

PubMed Abstract: Archaea of the order Thermoplasmatales push the boundaries of our current knowledge of prokaryotic life. They show distinct cellular plasticity, heterogenous cell morphologies, and lack a paracrystalline S-layer. As the S-layer has previously been implicated in acting as a stator scaffold for filaments driving cellular propulsion, particularly archaella, we asked whether the absence of an S-layer precludes the formation of functional archaella or pili in Thermoplasmatales. Using cryoEM, we investigated the two Thermoplasmatales species Cuniculiplasma divulgatum and Oxyplasma meridianum. We found that these species indeed generate pili and archaella and that the latter likely function in cellular propulsion. Whereas C. divulgatum produces pili with terminal hooks using a unique assembly machinery, O. meridianum generates unusually wide, "barbed" archaella with a high degree of glycosylation. Our results show that for the generation of functional archaella and pili, a canonical S-layer is not necessary.

PubMed: 40801261
DOI: 10.1093/ismejo/wraf176

Experimental method

ELECTRON MICROSCOPY (2.61 Å)