8RAO

Structure of Sen1-ADP.BeF3-RNA complex

Summary for 8RAO

• Entry DOI: 10.2210/pdb8rao/pdb
• EMDB information: 19021
• Descriptor: Helicase SEN1, RNA, ADENOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: rna polymerase ii, pol ii, termination, sen1, gene regulation
• Biological source: Saccharomyces cerevisiae (brewer's yeast); More
• Total number of polymer chains: 2
• Total formula weight: 264636.22

Authors

Rengachari, S.,Lidscreiber, M.,Cramer, P. (deposition date: 2023-12-01, release date: 2024-10-30, Last modification date: 2025-02-26)

Primary citation

Rengachari, S.,Hainthaler, T.,Oberthuer, C.,Lidschreiber, M.,Cramer, P.
Mechanism of polyadenylation-independent RNA polymerase II termination.
Nat.Struct.Mol.Biol., 32:339-345, 2025

PubMed Abstract: The mechanisms underlying the initiation and elongation of RNA polymerase II (Pol II) transcription are well-studied, whereas termination remains poorly understood. Here we analyze the mechanism of polyadenylation-independent Pol II termination mediated by the yeast Sen1 helicase. Cryo-electron microscopy structures of two pretermination intermediates show that Sen1 binds to Pol II and uses its adenosine triphosphatase activity to pull on exiting RNA in the 5' direction. This is predicted to push Pol II forward, induce an unstable hypertranslocated state and destabilize the transcription bubble, thereby facilitating termination. This mechanism of transcription termination may be widely used because it is conceptually conserved in the bacterial transcription system.

PubMed: 39424994
DOI: 10.1038/s41594-024-01409-0

Experimental method

ELECTRON MICROSCOPY (4.4 Å)