8RAM
Structure of Sen1 bound RNA Polymerase II pre-termination complex
Summary for 8RAM
| Entry DOI | 10.2210/pdb8ram/pdb |
| EMDB information | 19019 |
| Descriptor | DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (21 entities in total) |
| Functional Keywords | rna polymerase ii, pol ii, termination, sen1, gene regulation |
| Biological source | Saccharomyces cerevisiae (brewer's yeast) More |
| Total number of polymer chains | 19 |
| Total formula weight | 957838.99 |
| Authors | Rengachari, S.,Lidscreiber, M.,Cramer, P. (deposition date: 2023-12-01, release date: 2024-10-30, Last modification date: 2025-02-26) |
| Primary citation | Rengachari, S.,Hainthaler, T.,Oberthuer, C.,Lidschreiber, M.,Cramer, P. Mechanism of polyadenylation-independent RNA polymerase II termination. Nat.Struct.Mol.Biol., 32:339-345, 2025 Cited by PubMed Abstract: The mechanisms underlying the initiation and elongation of RNA polymerase II (Pol II) transcription are well-studied, whereas termination remains poorly understood. Here we analyze the mechanism of polyadenylation-independent Pol II termination mediated by the yeast Sen1 helicase. Cryo-electron microscopy structures of two pretermination intermediates show that Sen1 binds to Pol II and uses its adenosine triphosphatase activity to pull on exiting RNA in the 5' direction. This is predicted to push Pol II forward, induce an unstable hypertranslocated state and destabilize the transcription bubble, thereby facilitating termination. This mechanism of transcription termination may be widely used because it is conceptually conserved in the bacterial transcription system. PubMed: 39424994DOI: 10.1038/s41594-024-01409-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.8 Å) |
Structure validation
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