8R9R

GDP-bound state of S. putrefaciens FlhF

Summary for 8R9R

• Entry DOI: 10.2210/pdb8r9r/pdb
• Descriptor: Flagellar biosynthesis protein FlhF, GUANOSINE-5'-DIPHOSPHATE (3 entities in total)
• Functional Keywords: flagellum, assembly, motility, gtp-binding, gtpase, signaling protein
• Biological source: Shewanella putrefaciens
• Total number of polymer chains: 1
• Total formula weight: 32732.08

Authors

Mais, C.N.,Dornes, A.,Bange, G. (deposition date: 2023-11-30, release date: 2024-03-20)

Primary citation

Dornes, A.,Mais, C.N.,Bange, G.
Structure of the GDP-bound state of the SRP GTPase FlhF.
Acta Crystallogr.,Sect.F, 80:53-58, 2024

PubMed Abstract: The GTPase FlhF, a signal recognition particle (SRP)-type enzyme, is pivotal for spatial-numerical control and bacterial flagella assembly across diverse species, including pathogens. This study presents the X-ray structure of FlhF in its GDP-bound state at a resolution of 2.28 Å. The structure exhibits the classical N- and G-domain fold, consistent with related SRP GTPases such as Ffh and FtsY. Comparative analysis with GTP-loaded FlhF elucidates the conformational changes associated with GTP hydrolysis. These topological reconfigurations are similarly evident in Ffh and FtsY, and play a pivotal role in regulating the functions of these hydrolases.

PubMed: 38376823
DOI: 10.1107/S2053230X24000979

Experimental method

X-RAY DIFFRACTION (2.42 Å)