8R72
Polysaccharide lyase BtPL33HA (BT4410) Y291A with HA dp4 collected at 1.33 A
Summary for 8R72
| Entry DOI | 10.2210/pdb8r72/pdb |
| Descriptor | Heparinase, beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-beta-D-glucopyranuronic acid-(1-3)-2-acetamido-2-deoxy-beta-D-glucopyranose, ZINC ION, ... (4 entities in total) |
| Functional Keywords | polysaccharide lyase, glycosaminoglycan, hyaluronic acid, conformational change, lyase |
| Biological source | Bacteroides thetaiotaomicron VPI-5482 |
| Total number of polymer chains | 2 |
| Total formula weight | 148821.02 |
| Authors | Cartmell, A. (deposition date: 2023-11-23, release date: 2024-12-04, Last modification date: 2025-03-05) |
| Primary citation | Loiodice, M.,Drula, E.,McIver, Z.,Antonyuk, S.,Basle, A.,Lima, M.,Yates, E.A.,Byrne, D.P.,Coughlan, J.,Leech, A.,Mesdaghi, S.,Rigden, D.J.,Drouillard, S.,Helbert, W.,Henrissat, B.,Terrapon, N.,Wright, G.S.A.,Couturier, M.,Cartmell, A. Bacterial polysaccharide lyase family 33: Specificity from an evolutionarily conserved binding tunnel. Proc.Natl.Acad.Sci.USA, 122:e2421623122-e2421623122, 2025 Cited by PubMed Abstract: Acidic glycans are essential for the biology of multicellular eukaryotes. To utilize them, microbial life including symbionts and pathogens has evolved polysaccharide lyases (PL) that cleave their 1,4 glycosidic linkages via a β-elimination mechanism. PL family 33 (PL33) enzymes have the unusual ability to target a diverse range of glycosaminoglycans (GAGs), as well as the bacterial polymer, gellan gum. In order to gain more detailed insight into PL33 activities we recombinantly expressed 10 PL33 members derived from all major environments and further elucidated the detailed biochemical and biophysical properties of five, showing that their substrate specificity is conferred by variations in tunnel length and topography. The key amino acids involved in catalysis and substrate interactions were identified, and employing a combination of complementary biochemical, structural, and modeling approaches, we show that the tunnel topography is induced by substrate binding to the glycan. Structural and bioinformatic analyses revealed that these features are conserved across several lyase families as well as in mammalian GAG epimerases. PubMed: 39932998DOI: 10.1073/pnas.2421623122 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.58 Å) |
Structure validation
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