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8QZU

XhpG hydrolase mutant S98A of Xenorhabdus hominickii

Summary for 8QZU
Entry DOI10.2210/pdb8qzu/pdb
DescriptorCarboxylesterase, ACETATE ION (3 entities in total)
Functional Keywordsalpha/beta hydrolase, post-nrps enzyme, tailoring enzyme, oxopyrrolizidinacetamide hydrolase, biosynthetic protein
Biological sourceXenorhabdus hominickii
Total number of polymer chains1
Total formula weight29418.48
Authors
Calderari, A.,Gruez, A.,Weissman, K.J. (deposition date: 2023-10-30, release date: 2024-11-06)
Primary citationEffert, J.,Westphalen, M.,Calderari, A.,Shi, Y.M.,Elamri, I.,Najah, S.,Grun, P.,Li, Y.,Gruez, A.,Weissman, K.J.,Bode, H.B.
Pyrrolizwilline, a unique bacterial alkaloid assembled by a nonribosomal peptide synthetase and non-enzymatic dimerization.
Angew.Chem.Int.Ed.Engl., :e202411258-e202411258, 2024
Cited by
PubMed Abstract: Pyrrolizidine alkaloids (PAs) are a structurally diverse group of heterocyclic specialized metabolites characterized by a core structure comprising a hexahydro-1H-pyrrolizine. PAs are synthesized through two main pathways. In plants, assembly occurs via a homospermidine synthase, and in bacteria, through combined action of a nonribosomal peptide synthetase and a Baeyer-Villiger monooxygenase. While the toxic properties of plant-derived PAs and their prevalence in animal and human foods have been extensively studied, the biological roles and biosynthesis of more complex bacterial PAs are not well understood. Here, we report the identification and characterization of a bacterial biosynthetic gene cluster from Xenorhabdus hominickii, xhpA-G, which is responsible for producing the PA pseudo-dimer pyrrolizwilline. Analysis of X. hominickii promoter exchange mutants together with heterologous expression of xhpA-G in E. coli, revealed a set of pathway intermediates, two of which were chemically synthesized, as well as multiple derivatives. This information was leveraged to propose a detailed biosynthetic pathway to pyrrolizwilline. Furthermore, we have characterized the hydrolase XhpG, the key enzyme in the conversion of the pathway intermediate pyrrolizixenamide to pyrrolizwilline, using X-ray crystallography and small-angle X-ray scattering (SAXS).
PubMed: 39428351
DOI: 10.1002/anie.202411258
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.18 Å)
Structure validation

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