8QYT
Human Pyridoxine-5'-phosphate oxidase in complex with PLP
Summary for 8QYT
| Entry DOI | 10.2210/pdb8qyt/pdb |
| Descriptor | Pyridoxine-5'-phosphate oxidase, BETA-MERCAPTOETHANOL, FLAVIN MONONUCLEOTIDE, ... (6 entities in total) |
| Functional Keywords | oxidoreductase, pyridoxine biosynthesis, flavoprotein, pyridoxal phosphate |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 1 |
| Total formula weight | 31115.90 |
| Authors | |
| Primary citation | Barile, A.,Graziani, C.,Antonelli, L.,Parroni, A.,Fiorillo, A.,di Salvo, M.L.,Ilari, A.,Giorgi, A.,Rosignoli, S.,Paiardini, A.,Contestabile, R.,Tramonti, A. Identification of the pyridoxal 5'-phosphate allosteric site in human pyridox(am)ine 5'-phosphate oxidase. Protein Sci., 33:e4900-e4900, 2024 Cited by PubMed Abstract: Adequate levels of pyridoxal 5'-phosphate (PLP), the catalytically active form of vitamin B , and its proper distribution in the body are essential for human health. The PLP recycling pathway plays a crucial role in these processes and its defects cause severe neurological diseases. The enzyme pyridox(am)ine 5'-phosphate oxidase (PNPO), whose catalytic action yields PLP, is one of the key players in this pathway. Mutations in the gene encoding PNPO are responsible for a severe form of neonatal epilepsy. Recently, PNPO has also been described as a potential target for chemotherapeutic agents. Our laboratory has highlighted the crucial role of PNPO in the regulation of PLP levels in the cell, which occurs via a feedback inhibition mechanism of the enzyme, exerted by binding of PLP at an allosteric site. Through docking analyses and site-directed mutagenesis experiments, here we identified the allosteric PLP binding site of human PNPO. This site is located in the same protein region as the allosteric site we previously identified in the Escherichia coli enzyme homologue. However, the identity and arrangement of the amino acid residues involved in PLP binding are completely different and resemble those of the active site of PLP-dependent enzymes. The identification of the PLP allosteric site of human PNPO paves the way for the rational design of enzyme inhibitors as potential anti-cancer compounds. PubMed: 38284493DOI: 10.1002/pro.4900 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.69 Å) |
Structure validation
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