8QQJ
CryoEM structure of the type IV pilin PilA5 from Thermus thermophilus
Summary for 8QQJ
| Entry DOI | 10.2210/pdb8qqj/pdb |
| EMDB information | 18593 |
| Descriptor | Type IV narrow pilus major component PilA5, 7-Acetamido-5-acetimidoyl-3,5,7,9-tetradeoxy-L-glycero-L-manno-nonulosonic aci-(1-4)-alpha-D-mannopyranose-(1-3)-2-acetamido-2-deoxy-alpha-D-galactopyranose-(1-3)-2-acetamido-2-deoxy-alpha-D-galactopyranose, MAGNESIUM ION (3 entities in total) |
| Functional Keywords | type iv pilin glycosylation, twitching motility, protein fibril |
| Biological source | Thermus thermophilus HB27 |
| Total number of polymer chains | 31 |
| Total formula weight | 383406.95 |
| Authors | Gold, V.A.M.,Neuhaus, A.,Gaines, M.,Isupov, M.,McLaren, M. (deposition date: 2023-10-04, release date: 2024-10-16, Last modification date: 2026-04-22) |
| Primary citation | Neuhaus, A.,McLaren, M.,Isupov, M.N.,Gaines, M.,Buzzard, E.,Sikora, M.,Hanus, C.,Daum, B.,Averhoff, B.,Gold, V.A.M. Structural insights into the Thermus thermophilus type IV pilus machinery assembling two distinct pili. Commun Biol, 9:-, 2026 Cited by PubMed Abstract: Type IV pili are long, filamentous structures that extend from bacterial cell surfaces, enabling cells to respond to changing environments and facilitating genome plasticity. Thermus thermophilus HB27 produces two different type IV pili, each exhibiting distinct structural and functional properties. Here, we combine cryo-electron tomography, mutagenesis, and AlphaFold predictions to generate hypothetical in situ models of the T. thermophilus type IV pilus assembly machinery. Using single-particle cryo-electron microscopy, we determine structures of both filament types, enabling modelling of their surface glycans. Molecular dynamics simulations further reveal the flexibility of these glycans on extrusion. Integration of the filament structures with our hypothetical model of the assembly machinery offers a framework for further dissecting T4P architecture and biogenesis. PubMed: 41917195DOI: 10.1038/s42003-026-09762-0 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.63 Å) |
Structure validation
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