8QQE

Crystal structure of the complex between DMC1 and the PhePP domain of BRCA2

8QQE の概要

• エントリーDOI: 10.2210/pdb8qqe/pdb
• 分子名称: Meiotic recombination protein DMC1/LIM15 homolog, Breast cancer type 2 susceptibility protein, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: dna binding protein, meiotic recombination, ring protein, octamer, aaa atpase, recombination
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 80046.41

構造登録者

Miron, S.,Legrand, P.,Zinn-Justin, S. (登録日: 2023-10-04, 公開日: 2024-06-19, 最終更新日: 2024-07-17)

主引用文献

Miron, S.,Legrand, P.,Dupaigne, P.,van Rossum-Fikkert, S.E.,Ristic, D.,Majeed, A.,Kanaar, R.,Zinn-Justin, S.,Zelensky, A.N.
DMC1 and RAD51 bind FxxA and FxPP motifs of BRCA2 via two separate interfaces.
Nucleic Acids Res., 52:7337-7353, 2024

PubMed Abstract: In vertebrates, the BRCA2 protein is essential for meiotic and somatic homologous recombination due to its interaction with the RAD51 and DMC1 recombinases through FxxA and FxPP motifs (here named A- and P-motifs, respectively). The A-motifs present in the eight BRC repeats of BRCA2 compete with the A-motif of RAD51, which is responsible for its self-oligomerization. BRCs thus disrupt RAD51 nucleoprotein filaments in vitro. The role of the P-motifs is less studied. We recently found that deletion of Brca2 exons 12-14 encoding one of them (the prototypical 'PhePP' motif), disrupts DMC1 but not RAD51 function in mouse meiosis. Here we provide a mechanistic explanation for this phenotype by solving the crystal structure of the complex between a BRCA2 fragment containing the PhePP motif and DMC1. Our structure reveals that, despite sharing a conserved phenylalanine, the A- and P-motifs bind to distinct sites on the ATPase domain of the recombinases. The P-motif interacts with a site that is accessible in DMC1 octamers and nucleoprotein filaments. Moreover, we show that this interaction also involves the adjacent protomer and thus increases the stability of the DMC1 nucleoprotein filaments. We extend our analysis to other P-motifs from RAD51AP1 and FIGNL1.

PubMed: 38828772
DOI: 10.1093/nar/gkae452

実験手法

X-RAY DIFFRACTION (3.461 Å)