8QQ3

Streptavidin with a Ni-cofactor

8QQ3 の概要

• エントリーDOI: 10.2210/pdb8qq3/pdb
• 分子名称: Streptavidin, 4-[4-[(3~{a}~{S},4~{S},6~{a}~{R})-2-oxidanylidene-1,3,3~{a},4,6,6~{a}-hexahydrothieno[3,4-d]imidazol-4-yl]butylamino]-~{N}1,~{N}1'-di(quinolin-8-yl)cyclohexane-1,1-dicarboxamide, NICKEL (II) ION, ... (4 entities in total)
• 機能のキーワード: artificial metalloenzymes, metal binding protein
• 由来する生物種: Streptomyces avidinii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 69003.38

構造登録者

Zhang, K.,Jakob, R.P.,Ward, T.R. (登録日: 2023-10-03, 公開日: 2024-02-07, 最終更新日: 2024-02-21)

主引用文献

Yu, K.,Zhang, K.,Jakob, R.P.,Maier, T.,Ward, T.R.
An artificial nickel chlorinase based on the biotin-streptavidin technology.
Chem.Commun.(Camb.), 60:1944-1947, 2024

PubMed Abstract: Herein, we report on an artificial nickel chlorinase (ANCase) resulting from anchoring a biotinylated nickel-based cofactor within streptavidin (Sav). The resulting ANCase was efficient for the chlorination of diverse C(sp)-H bonds. Guided by the X-ray analysis of the ANCase, the activity of the artificial chlorinase could be significantly improved. This approach opens interesting perspectives for late-stage functionalization of organic intermediates as it complements biocatalytic chlorination strategies.

PubMed: 38277163
DOI: 10.1039/d3cc05847f

実験手法

X-RAY DIFFRACTION (1.6 Å)