8QOI
Structure of the human 80S ribosome at 1.9 A resolution - the molecular role of chemical modifications and ions in RNA
This is a non-PDB format compatible entry.
Summary for 8QOI
| Entry DOI | 10.2210/pdb8qoi/pdb |
| EMDB information | 18539 |
| Descriptor | 28S rRNA (3773-MER), 60S ribosomal protein L7a, 60S ribosomal protein L9, ... (84 entities in total) |
| Functional Keywords | ribosome, cryo-electron microscopie, post-transcriptional modifications |
| Biological source | Homo sapiens More |
| Total number of polymer chains | 79 |
| Total formula weight | 3790357.17 |
| Authors | Holvec, S.,Barchet, C.,Frechin, L.,Hazemann, I.,von Loeffelholz, O.,Klaholz, B.P. (deposition date: 2023-09-29, release date: 2024-06-12, Last modification date: 2024-08-28) |
| Primary citation | Holvec, S.,Barchet, C.,Lechner, A.,Frechin, L.,De Silva, S.N.T.,Hazemann, I.,Wolff, P.,von Loeffelholz, O.,Klaholz, B.P. The structure of the human 80S ribosome at 1.9 angstrom resolution reveals the molecular role of chemical modifications and ions in RNA. Nat.Struct.Mol.Biol., 31:1251-1264, 2024 Cited by PubMed Abstract: The ribosomal RNA of the human protein synthesis machinery comprises numerous chemical modifications that are introduced during ribosome biogenesis. Here we present the 1.9 Å resolution cryo electron microscopy structure of the 80S human ribosome resolving numerous new ribosomal RNA modifications and functionally important ions such as Zn, K and Mg, including their associated individual water molecules. The 2'-O-methylation, pseudo-uridine and base modifications were confirmed by mass spectrometry, resulting in a complete investigation of the >230 sites, many of which could not be addressed previously. They choreograph key interactions within the RNA and at the interface with proteins, including at the ribosomal subunit interfaces of the fully assembled 80S ribosome. Uridine isomerization turns out to be a key mechanism for U-A base pair stabilization in RNA in general. The structural environment of chemical modifications and ions is primordial for the RNA architecture of the mature human ribosome, hence providing a structural framework to address their role in healthy states and in human diseases. PubMed: 38844527DOI: 10.1038/s41594-024-01274-x PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (1.9 Å) |
Structure validation
Download full validation report
