8QHM

DNA mimic Foldamer with sticky ends

8QHM の概要

• エントリーDOI: 10.2210/pdb8qhm/pdb
• 分子名称: DNA mimic Foldamer (1 entity in total)
• 機能のキーワード: dna mimic, unknown function
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 9230.31

構造登録者

Deepak, D.,Loos, M.,Huc, I. (登録日: 2023-09-08, 公開日: 2023-10-11, 最終更新日: 2024-09-04)

主引用文献

Corvaglia, V.,Wu, J.,Deepak, D.,Loos, M.,Huc, I.
Enhancing the Features of DNA Mimic Foldamers for Structural Investigations.
Chemistry, 30:e202303650-e202303650, 2024

PubMed Abstract: DNA mimic foldamers based on aromatic oligoamide helices bearing anionic phosphonate side chains have been shown to bind to DNA-binding proteins sometimes orders of magnitude better than DNA itself. Here, we introduce new features in the DNA mimic foldamers to facilitate structural investigations of their interactions with proteins. Thirteen new foldamer sequences have been synthesized and characterized using NMR, circular dichroism, molecular modeling, and X-ray crystallography. The results show that foldamer helix handedness can be quantitatively biased by means of a single stereogenic center, that the foldamer structure can be made C-symmetrical as in palindromic B-DNA sequences, and that associations between foldamer helices can be promoted utilizing dedicated C-terminal residues that act as sticky ends in B-DNA structures.

PubMed: 38193643
DOI: 10.1002/chem.202303650

実験手法

X-RAY DIFFRACTION (3 Å)