8QEP
RNA polymerase II bound to Alu RNA right arm
Summary for 8QEP
| Entry DOI | 10.2210/pdb8qep/pdb |
| EMDB information | 18375 |
| Descriptor | DNA-directed RNA polymerase subunit, DNA-directed RNA polymerase II subunit RPB11-a, RNA polymerase II, I and III subunit K, ... (12 entities in total) |
| Functional Keywords | transcription inhibition, noncoding rna, rna polymerase ii, alu rna, transcription |
| Biological source | Sus scrofa domesticus (domestic pig) More |
| Total number of polymer chains | 11 |
| Total formula weight | 501314.96 |
| Authors | Tluckova, K.,Bernecky, C. (deposition date: 2023-09-01, release date: 2025-01-22, Last modification date: 2025-04-23) |
| Primary citation | Tluckova, K.,Kaczmarek, B.,Salmazo, A.,Bernecky, C. Mechanism of mammalian transcriptional repression by noncoding RNA. Nat.Struct.Mol.Biol., 32:607-612, 2025 Cited by PubMed Abstract: Transcription by RNA polymerase II (Pol II) can be repressed by noncoding RNA, including the human RNA Alu. However, the mechanism by which endogenous RNAs repress transcription remains unclear. Here we present cryogenic-electron microscopy structures of Pol II bound to Alu RNA, which reveal that Alu RNA mimics how DNA and RNA bind to Pol II during transcription elongation. Further, we show how distinct domains of the general transcription factor TFIIF control repressive activity. Together, we reveal how a noncoding RNA can regulate mammalian gene expression. PubMed: 39762629DOI: 10.1038/s41594-024-01448-7 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.5 Å) |
Structure validation
Download full validation report
