8Q91

Structure of the human 20S U5 snRNP core

Summary for 8Q91

Entry DOI	10.2210/pdb8q91/pdb
EMDB information	18267
Descriptor	CD2 antigen cytoplasmic tail-binding protein 2, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, ... (15 entities in total)
Functional Keywords	snrnp, cd2bp2, spliceosome, u5, splicing
Biological source	Homo sapiens (human) More
Total number of polymer chains	14
Total formula weight	1001205.95
Authors	Schneider, S.,Galej, W.P. (deposition date: 2023-08-19, release date: 2024-03-27, Last modification date: 2024-11-06)
Primary citation	Schneider, S.,Brandina, I.,Peter, D.,Lagad, S.,Fraudeau, A.,Portell-Montserrat, J.,Tholen, J.,Zhao, J.,Galej, W.P. Structure of the human 20S U5 snRNP. Nat.Struct.Mol.Biol., 31:752-756, 2024 Cited by PubMed Abstract: The 20S U5 small nuclear ribonucleoprotein particle (snRNP) is a 17-subunit RNA-protein complex and a precursor of the U4/U6.U5 tri-snRNP, the major building block of the precatalytic spliceosome. CD2BP2 is a hallmark protein of the 20S U5 snRNP, absent from the mature tri-snRNP. Here we report a high-resolution cryogenic electron microscopy structure of the 20S U5 snRNP, shedding light on the mutually exclusive interfaces utilized during tri-snRNP assembly and the role of the CD2BP2 in facilitating this process. PubMed: 38467877 DOI: 10.1038/s41594-024-01250-5 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.1 Å)

Structure validation