8Q85

Outer kinetochore Dam1 protomer monomer Ndc80-Nuf2 coiled-coil complex

Summary for 8Q85

• Entry DOI: 10.2210/pdb8q85/pdb
• EMDB information: 18247
• Descriptor: Kinetochore protein NDC80, DASH complex subunit ASK1, DASH complex subunit HSK3, ... (12 entities in total)
• Functional Keywords: kinetochore, microtubule, error correction, chromosome segregation, cell cycle
• Biological source: Saccharomyces cerevisiae (baker's yeast); More
• Total number of polymer chains: 12
• Total formula weight: 337533.02

Authors

Muir, K.W.,Barford, D. (deposition date: 2023-08-17, release date: 2023-12-06, Last modification date: 2023-12-20)

Primary citation

Muir, K.W.,Batters, C.,Dendooven, T.,Yang, J.,Zhang, Z.,Burt, A.,Barford, D.
Structural mechanism of outer kinetochore Dam1-Ndc80 complex assembly on microtubules.
Science, 382:1184-1190, 2023

PubMed Abstract: Kinetochores couple chromosomes to the mitotic spindle to segregate the genome during cell division. An error correction mechanism drives the turnover of kinetochore-microtubule attachments until biorientation is achieved. The structural basis for how kinetochore-mediated chromosome segregation is accomplished and regulated remains an outstanding question. In this work, we describe the cryo-electron microscopy structure of the budding yeast outer kinetochore Ndc80 and Dam1 ring complexes assembled onto microtubules. Complex assembly occurs through multiple interfaces, and a staple within Dam1 aids ring assembly. Perturbation of key interfaces suppresses yeast viability. Force-rupture assays indicated that this is a consequence of impaired kinetochore-microtubule attachment. The presence of error correction phosphorylation sites at Ndc80-Dam1 ring complex interfaces and the Dam1 staple explains how kinetochore-microtubule attachments are destabilized and reset.

PubMed: 38060647
DOI: 10.1126/science.adj8736

Experimental method

ELECTRON MICROSCOPY (3.97 Å)