8Q7Q

Structure of the recycling U5 snRNP bound to chaperones CD2BP2 and TSSC4 (State 2)

Summary for 8Q7Q

• Entry DOI: 10.2210/pdb8q7q/pdb
• Related: 8Q7V 8Q7W 8Q7X
• EMDB information: 18229 18238 18239
• Descriptor: U5 snRNA, Small nuclear ribonucleoprotein-associated proteins B and B', Small nuclear ribonucleoprotein Sm D2, ... (17 entities in total)
• Functional Keywords: u5 snrnp, cd2bp2, tssc4, spliceosome, splicing
• Biological source: Homo sapiens; More
• Total number of polymer chains: 15
• Total formula weight: 979325.23

Authors

Riabov Bassat, D.,Plaschka, C.,Vorlaender, M.K. (deposition date: 2023-08-16, release date: 2024-04-17, Last modification date: 2024-05-29)

Primary citation

Riabov Bassat, D.,Visanpattanasin, S.,Vorlander, M.K.,Fin, L.,Phillips, A.W.,Plaschka, C.
Structural basis of human U5 snRNP late biogenesis and recycling.
Nat.Struct.Mol.Biol., 31:747-751, 2024

PubMed Abstract: Pre-mRNA splicing by the spliceosome requires the biogenesis and recycling of its small nuclear ribonucleoprotein (snRNP) complexes, which are consumed in each round of splicing. The human U5 snRNP is the ~1 MDa 'heart' of the spliceosome and is recycled through an unknown mechanism involving major architectural rearrangements and the dedicated chaperones CD2BP2 and TSSC4. Late steps in U5 snRNP biogenesis similarly involve these chaperones. Here we report cryo-electron microscopy structures of four human U5 snRNP-CD2BP2-TSSC4 complexes, revealing how a series of molecular events primes the U5 snRNP to generate the ~2 MDa U4/U6.U5 tri-snRNP, the largest building block of the spliceosome.

PubMed: 38467876
DOI: 10.1038/s41594-024-01243-4

Experimental method

ELECTRON MICROSCOPY (3.2 Å)