8Q79

Structure of mBaoJin at pH 6.5

Summary for 8Q79

• Entry DOI: 10.2210/pdb8q79/pdb
• Descriptor: mBaoJin, CHLORIDE ION (3 entities in total)
• Functional Keywords: mbaojin, fluorescent protein
• Biological source: Cytaeis uchidae
• Total number of polymer chains: 2
• Total formula weight: 53753.15

Authors

Samygina, V.R.,Subach, O.M.,Vlaskina, A.V.,Nikolaeva, A.Y.,Borshchevsky, V.,Qin, W.,Subach, F.V. (deposition date: 2023-08-15, release date: 2023-12-27, Last modification date: 2024-10-16)

Primary citation

Zhang, H.,Lesnov, G.D.,Subach, O.M.,Zhang, W.,Kuzmicheva, T.P.,Vlaskina, A.V.,Samygina, V.R.,Chen, L.,Ye, X.,Nikolaeva, A.Y.,Gabdulkhakov, A.,Papadaki, S.,Qin, W.,Borshchevskiy, V.,Perfilov, M.M.,Gavrikov, A.S.,Drobizhev, M.,Mishin, A.S.,Piatkevich, K.D.,Subach, F.V.
Bright and stable monomeric green fluorescent protein derived from StayGold.
Nat.Methods, 21:657-665, 2024

PubMed Abstract: The high brightness and photostability of the green fluorescent protein StayGold make it a particularly attractive probe for long-term live-cell imaging; however, its dimeric nature precludes its application as a fluorescent tag for some proteins. Here, we report the development and crystal structures of a monomeric variant of StayGold, named mBaoJin, which preserves the beneficial properties of its precursor, while serving as a tag for structural proteins and membranes. Systematic benchmarking of mBaoJin against popular green fluorescent proteins and other recently introduced monomeric and pseudomonomeric derivatives of StayGold established mBaoJin as a bright and photostable fluorescent protein, exhibiting rapid maturation and high pH/chemical stability. mBaoJin was also demonstrated for super-resolution, long-term live-cell imaging and expansion microscopy. We further showed the applicability of mBaoJin for neuronal labeling in model organisms, including Caenorhabditis elegans and mice.

PubMed: 38409224
DOI: 10.1038/s41592-024-02203-y

Experimental method

X-RAY DIFFRACTION (1.45 Å)