8PXZ
Crystal structure of the transpeptidase LdtMt2 from Mycobacterium tuberculosis in complex with natural substrate
Summary for 8PXZ
| Entry DOI | 10.2210/pdb8pxz/pdb |
| Descriptor | L,D-transpeptidase 2, Peptidoglycan tripeptide, Peptidoglycan dipeptide, ... (7 entities in total) |
| Functional Keywords | ldtmt2, substrate complex, mycobacterium tuberculosis, l, d-transpeptidase, antimicrobial protein |
| Biological source | Mycobacterium tuberculosis More |
| Total number of polymer chains | 3 |
| Total formula weight | 38901.52 |
| Authors | de Munnik, M.,Schofield, C.J. (deposition date: 2023-07-24, release date: 2024-08-07, Last modification date: 2024-10-16) |
| Primary citation | de Munnik, M.,Lang, P.A.,Calvopina, K.,Rabe, P.,Brem, J.,Schofield, C.J. Biochemical and crystallographic studies of L,D-transpeptidase 2 from Mycobacterium tuberculosis with its natural monomer substrate. Commun Biol, 7:1173-1173, 2024 Cited by PubMed Abstract: The essential L,D-transpeptidase of Mycobacterium tuberculosis (Ldt) catalyses the formation of 3 3 cross-links in cell wall peptidoglycan and is a target for development of antituberculosis therapeutics. Efforts to inhibit Ldt have been hampered by lack of knowledge of how it binds its substrate. To address this gap, we optimised the isolation of natural disaccharide tetrapeptide monomers from the Corynebacterium jeikeium bacterial cell wall through overproduction of the peptidoglycan sacculus. The tetrapeptides were used in binding / turnover assays and biophysical studies on Ldt We determined a crystal structure of wild-type Ldt reacted with its natural substrate, the tetrapeptide monomer of the peptidoglycan layer. This structure shows formation of a thioester linking the catalytic cysteine and the donor substrate, reflecting an intermediate in the transpeptidase reaction; it informs on the mode of entrance of the donor substrate into the Ldt active site. The results will be useful in design of Ldt inhibitors, including those based on substrate binding interactions, a strategy successfully employed for other nucleophilic cysteine enzymes. PubMed: 39294212DOI: 10.1038/s42003-024-06785-3 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.98 Å) |
Structure validation
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