8PPU

Pyrococcus abyssi DNA polymerase D (PolD) in its editing mode bound to a primer/template substrate containing three consecutive mismatches

Summary for 8PPU

• Entry DOI: 10.2210/pdb8ppu/pdb
• EMDB information: 17816
• Descriptor: DNA (5'-D(P*CP*CP*GP*GP*GP*CP*CP*GP*AP*GP*CP*CP*GP*TP*(GS)P*(C7R)P*(PST)P*(PST)P*(PST))-3'), DNA (5'-D(P*AP*GP*CP*AP*CP*GP*GP*CP*TP*CP*GP*GP*CP*CP*CP*GP*G)-3'), DNA polymerase II small subunit, ... (7 entities in total)
• Functional Keywords: polymerase, dna, replication, pold, archaea, editing, proofreading, exonuclease, nuclease, dna binding protein
• Biological source: Pyrococcus abyssi GE5; More
• Total number of polymer chains: 7
• Total formula weight: 321343.53

Authors

Betancurt-Anzola, L.,Martinez-Carranza, M.,Zatopek, K.M.,Gardner, A.F.,Sauguet, L. (deposition date: 2023-07-10, release date: 2023-12-20, Last modification date: 2023-12-27)

• Primary citation: Betancurt-Anzola, L.,Martinez-Carranza, M.,Delarue, M.,Zatopek, K.M.,Gardner, A.F.,Sauguet, L.; Molecular basis for proofreading by the unique exonuclease domain of Family-D DNA polymerases.; Nat Commun, 14:8306-8306, 2023; PubMed: 38097591; DOI: 10.1038/s41467-023-44125-x

Experimental method

ELECTRON MICROSCOPY (3.02 Å)