8PPQ
Tick-borne encephalitis virus Kuutsalo-14 prM3E3 trimer
Summary for 8PPQ
| Entry DOI | 10.2210/pdb8ppq/pdb |
| EMDB information | 17808 |
| Descriptor | Envelope protein E, Protein prM, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | complex, spike, fusion protein, glycosylation, transmembrane protein, maturation, virus, trimer, hexamer, dimer |
| Biological source | Tick-borne encephalitis virus More |
| Total number of polymer chains | 6 |
| Total formula weight | 215568.44 |
| Authors | Anastasina, M.,Domanska, A.,Pulkkinen, L.I.A.,Butcher, S.J. (deposition date: 2023-07-07, release date: 2024-06-12, Last modification date: 2024-10-23) |
| Primary citation | Anastasina, M.,Fuzik, T.,Domanska, A.,Pulkkinen, L.I.A.,Smerdova, L.,Formanova, P.P.,Strakova, P.,Novacek, J.,Ruzek, D.,Plevka, P.,Butcher, S.J. The structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation. Sci Adv, 10:eadl1888-eadl1888, 2024 Cited by PubMed Abstract: We present structures of three immature tick-borne encephalitis virus (TBEV) isolates. Our atomic models of the major viral components, the E and prM proteins, indicate that the pr domains of prM have a critical role in holding the heterohexameric prM3E3 spikes in a metastable conformation. Destabilization of the prM furin-sensitive loop at acidic pH facilitates its processing. The prM topology and domain assignment in TBEV is similar to the mosquito-borne Binjari virus, but is in contrast to other immature flavivirus models. These results support that prM cleavage, the collapse of E protein ectodomains onto the virion surface, the large movement of the membrane domains of both E and M, and the release of the pr fragment from the particle render the virus mature and infectious. Our work favors the collapse model of flavivirus maturation warranting further studies of immature flaviviruses to determine the sequence of events and mechanistic details driving flavivirus maturation. PubMed: 38959313DOI: 10.1126/sciadv.adl1888 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.9 Å) |
Structure validation
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