8PP7

human RYBP-PRC1 bound to mononucleosome

8PP7 の概要

• エントリーDOI: 10.2210/pdb8pp7/pdb
• EMDBエントリー: 17797
• 分子名称: Polycomb complex protein BMI-1, E3 ubiquitin-protein ligase RING2, Histone H3 (Fragment), ... (9 entities in total)
• 機能のキーワード: ncprc1 complex, nucleosome, h2a, histones, ring1b, bmi1, heterodimer, e3 ligase, k119, gene regulation
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 413744.06

構造登録者

Ciapponi, M.,Benda, C.,Mueller, J. (登録日: 2023-07-06, 公開日: 2024-03-27, 最終更新日: 2025-07-09)

主引用文献

Ciapponi, M.,Karlukova, E.,Schkolziger, S.,Benda, C.,Muller, J.
Structural basis of the histone ubiquitination read-write mechanism of RYBP-PRC1.
Nat.Struct.Mol.Biol., 31:1023-1027, 2024

PubMed Abstract: Histone H2A monoubiquitination (H2Aub1) by the PRC1 subunit RING1B entails a positive feedback loop, mediated by the RING1B-interacting protein RYBP. We uncover that human RYBP-PRC1 binds unmodified nucleosomes via RING1B but H2Aub1-modified nucleosomes via RYBP. RYBP interactions with both ubiquitin and the nucleosome acidic patch create the high binding affinity that favors RYBP- over RING1B-directed PRC1 binding to H2Aub1-modified nucleosomes; this enables RING1B to monoubiquitinate H2A in neighboring unmodified nucleosomes.

PubMed: 38528151
DOI: 10.1038/s41594-024-01258-x

実験手法

ELECTRON MICROSCOPY (2.91 Å)