8POV

Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]-Hydrogenase from Cupriavidus necator in the H2-reduced state at 1.92 A Resolution.

Summary for 8POV

• Entry DOI: 10.2210/pdb8pov/pdb
• Related: 3RGW 4IUB 4IUC 4IUD 4TTT 5D51 5MDJ 5MDK 5MDL 7ODG 7ODH
• Descriptor: Uptake hydrogenase large subunit, Uptake hydrogenase small subunit, formyl[bis(hydrocyanato-1kappaC)]ironnickel(Fe-Ni), ... (7 entities in total)
• Functional Keywords: [nife]-hydrogenase, hydrogenase, oxygen-tolerance, hydrogen catalysis, knallgasbacteria, proteobacteria, metalloenzyme, bimetallic, ni-fe active site, [4fe-3s], proximal cluster, aerobic hydrogen bacteria, oxidoreductase, electron transfer, metalloprotein, catalytic center, membrane, membrane-bound, oxidoreductase-oxidoreductase complex, electron relay, cubane cluster, low potential, cluster tuning, electron transport, h2-reduced state
• Biological source: Cupriavidus necator H16; More
• Total number of polymer chains: 2
• Total formula weight: 104401.01

Authors

Schmidt, A.,Kalms, J.,Scheerer, P. (deposition date: 2023-07-05, release date: 2023-11-01)

• Primary citation: Schmidt, A.,Kalms, J.,Lorent, C.,Katz, S.,Frielingsdorf, S.,Evans, R.M.,Fritsch, J.,Siebert, E.,Teutloff, C.,Armstrong, F.A.,Zebger, I.,Lenz, O.,Scheerer, P.; Stepwise conversion of the Cys 6 [4Fe-3S] to a Cys 4 [4Fe-4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase.; Chem Sci, 14:11105-11120, 2023; PubMed: 37860641; DOI: 10.1039/d3sc03739h

Experimental method

X-RAY DIFFRACTION (1.92 Å)