8POU

Crystal Structure of the C19G/C120G variant of the membrane-bound [NiFe]-Hydrogenase from Cupriavidus necator in the air-oxidized state at 1.65 A Resolution.

Summary for 8POU

Entry DOI	10.2210/pdb8pou/pdb
Related	3RGW 4IUB 4IUC 4IUD 4TTT 5MDJ 5MDK 5MDL 7ODG 7ODH
Descriptor	Uptake hydrogenase large subunit, Uptake hydrogenase small subunit, NI-FE OXIDIZED ACTIVE CENTER, ... (8 entities in total)
Functional Keywords	[nife]-hydrogenase, hydrogenase, oxygen-tolerance, hydrogen catalysis, knallgasbacteria, proteobacteria, metalloenzyme, bimetallic, ni-fe active site, [4fe-3s], proximal cluster, aerobic hydrogen bacteria, oxidoreductase, electron transfer, metalloprotein, catalytic center, membrane, membrane-bound, oxidoreductase-oxidoreductase complex, electron relay, cubane cluster, low potential, cluster tuning, electron transport
Biological source	Cupriavidus necator H16 More
Total number of polymer chains	2
Total formula weight	104522.36
Authors	Schmidt, A.,Kalms, J.,Scheerer, P. (deposition date: 2023-07-05, release date: 2023-11-01, Last modification date: 2024-02-07)
Primary citation	Schmidt, A.,Kalms, J.,Lorent, C.,Katz, S.,Frielingsdorf, S.,Evans, R.M.,Fritsch, J.,Siebert, E.,Teutloff, C.,Armstrong, F.A.,Zebger, I.,Lenz, O.,Scheerer, P. Stepwise conversion of the Cys 6 [4Fe-3S] to a Cys 4 [4Fe-4S] cluster and its impact on the oxygen tolerance of [NiFe]-hydrogenase. Chem Sci, 14:11105-11120, 2023 Cited by PubMed: 37860641 DOI: 10.1039/d3sc03739h PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.65 Å)

Structure validation