8PNU
Cryo-EM structure of styrene oxide isomerase bound to benzylamine inhibitor
Summary for 8PNU
| Entry DOI | 10.2210/pdb8pnu/pdb |
| EMDB information | 17785 |
| Descriptor | Styrene oxide isomerase, Nanobody, BENZYLAMINE, ... (5 entities in total) |
| Functional Keywords | heme binding protein, enzyme, isomerase, membrane protein |
| Biological source | Pseudomonas sp. VLB120 More |
| Total number of polymer chains | 12 |
| Total formula weight | 206759.88 |
| Authors | Khanppnavar, B.,Korkhov, V.,Li, X. (deposition date: 2023-07-02, release date: 2024-04-03, Last modification date: 2024-10-23) |
| Primary citation | Khanppnavar, B.,Choo, J.P.S.,Hagedoorn, P.L.,Smolentsev, G.,Stefanic, S.,Kumaran, S.,Tischler, D.,Winkler, F.K.,Korkhov, V.M.,Li, Z.,Kammerer, R.A.,Li, X. Structural basis of the Meinwald rearrangement catalysed by styrene oxide isomerase. Nat.Chem., 16:1496-1504, 2024 Cited by PubMed Abstract: Membrane-bound styrene oxide isomerase (SOI) catalyses the Meinwald rearrangement-a Lewis-acid-catalysed isomerization of an epoxide to a carbonyl compound-and has been used in single and cascade reactions. However, the structural information that explains its reaction mechanism has remained elusive. Here we determine cryo-electron microscopy (cryo-EM) structures of SOI bound to a single-domain antibody with and without the competitive inhibitor benzylamine, and elucidate the catalytic mechanism using electron paramagnetic resonance spectroscopy, functional assays, biophysical methods and docking experiments. We find ferric haem b bound at the subunit interface of the trimeric enzyme through H58, where Fe(III) acts as the Lewis acid by binding to the epoxide oxygen. Y103 and N64 and a hydrophobic pocket binding the oxygen of the epoxide and the aryl group, respectively, position substrates in a manner that explains the high regio-selectivity and stereo-specificity of SOI. Our findings can support extending the range of epoxide substrates and be used to potentially repurpose SOI for the catalysis of new-to-nature Fe-based chemical reactions. PubMed: 38744914DOI: 10.1038/s41557-024-01523-y PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.12 Å) |
Structure validation
Download full validation report
